tag:blogger.com,1999:blog-5694894153899281485.post5061657831940970205..comments2024-03-09T08:14:49.856-08:00Comments on LymeMD: Western blot biasesLyme report: Montgomery County, MDhttp://www.blogger.com/profile/11035327980787631502noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-5694894153899281485.post-67696569539629944562013-04-22T19:50:10.918-07:002013-04-22T19:50:10.918-07:00Although this does not give any quantitative data ...Although this does not give any quantitative data unless proper controls are used...Anonymoushttps://www.blogger.com/profile/06602702918846411029noreply@blogger.comtag:blogger.com,1999:blog-5694894153899281485.post-50478859127193931922013-04-22T19:40:58.830-07:002013-04-22T19:40:58.830-07:00So this interesting, but as a graduate student who...So this interesting, but as a graduate student who runs western blots, the variability in the number of bands on the western can come from many things.<br /><br />First, you might see more bands from one westernblot compared to the other due to an increase time of transfer from the gel to the PVDF membrane. The proteins isolated from blood are run into the gel and transferred to the membrane. <br /><br />Secondly, the purity of the antibodies can be in question. Testing of the antibody can indicate if some bands are non-specific etc.<br /><br />Thirdly, I would expect to observe different protein bands on different days. Protein expression changes when you get a cold, when you get a pin prick in your finger, when you get a bruise, therefore I am not surprised that the number of bands changes.<br /><br />If you assume that the antibody is pure, then If you see the band, the protein is there. Anonymoushttps://www.blogger.com/profile/06602702918846411029noreply@blogger.comtag:blogger.com,1999:blog-5694894153899281485.post-89816540571323296482012-03-23T14:36:08.421-07:002012-03-23T14:36:08.421-07:00I womder if they use different strains in their te...I womder if they use different strains in their tests. <br /><br />Doesn't differing results from differing labs prove that denying treatment because of one negative result makes no sense?Pearlhttps://www.blogger.com/profile/09576144049302954879noreply@blogger.comtag:blogger.com,1999:blog-5694894153899281485.post-78635138785525399782012-03-16T06:52:54.161-07:002012-03-16T06:52:54.161-07:00Are there any reference standards against which to...Are there any reference standards against which to check the kit or in-house preparations? <br /><br />Thanks for doing these blogs!worriedmomhttps://www.blogger.com/profile/15793273940892928730noreply@blogger.com